Antibodies, A Laboratory Manual, Second Edition, Edited by Edward A. Greenfield



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Ridding Cells of Mycoplasma by Passage through Mice

(Protocol summary only for purposes of this preview site)

Growing hybridoma cells as ascites tumors is one of the most effective methods of removing mycoplasma contamination. The mycoplasmas are removed from the hybridoma cell surface by the immune system of the mouse. Mice must be of the same genetic background as the hybridomas. This technique is the same as the method for ridding cells of bacterial or fungal infection. Cells recovered from the ascites should be rescreened using one of the techniques described in Protocols 8 and 9. The success rate for this method is perhaps 80.

Antibodies: A Laboratory Manual, Second edition
Antibodies: A Laboratory Manual, Second edition
Antibodies: A Laboratory Manual, Second edition

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