Antibodies, A Laboratory Manual, Second Edition, Edited by Edward A. Greenfield



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Semi-Dry Electrophoretic Transfer

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Semi-dry electrophoretic transfer of proteins to the membrane was first introduced by Kyhse-Andersen (1984) and is now widely used by the scientific community because of its ease of use and high efficiency. Semi-dry electrophoretic transfer is the quickest way to efficiently transfer the proteins from polyacrylamide gels to the membrane. It works well for both nitrocellulose and PVDF membranes. The advantage of this method over the wet transfer method is speed and the significantly lower amount of buffer required for transfer. In addition, although temperature control is recommended for most wet transfer devices, it is not necessary for the semi-dry transfer systems. Semi-dry transfer does not require a large power source, and it can be adapted to handle stacks of gelmembrane sandwiches (up to six). In the original description, different buffers were described for the anode and cathode, but it was subsequently shown that a simple buffer system using a modification of the Tris/glycine SDSpolyacrylamide gel running buffer is just as effective and much easier.

Antibodies: A Laboratory Manual, Second edition
Antibodies: A Laboratory Manual, Second edition
Antibodies: A Laboratory Manual, Second edition

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