Antibodies, A Laboratory Manual, Second Edition, Edited by Edward A. Greenfield



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Immunizing Mice and Rats with Nitrocellulose-Bound Antigen

(Protocol summary only for purposes of this preview site)

A desired target antigen within a mixture of antigenic proteins like a cell lysate can be separated from the mixture by gel electrophoresis. Extracting the antigen from a section of gel and eluting it can be cumbersome and often results in significant loss of protein. Injecting the polyacrylamide gel piece containing the antigen may not be tolerated by the animal. Alternatively, as this protocol details, the protein can be transferred from the gel onto nitrocellulose and then injected either subcutaneously or into the peritoneal cavity.

Antibodies: A Laboratory Manual, Second edition
Antibodies: A Laboratory Manual, Second edition
Antibodies: A Laboratory Manual, Second edition

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