Antibodies, A Laboratory Manual, Second Edition, Edited by Edward A. Greenfield



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Antibody Capture on Permeabilized Whole Cells (Immunofluorescence)

(Protocol summary only for purposes of this preview site)

The procedure described in Protocol 7 can also be adapted to screen for antibodies to internal proteins by permeabilizing the cells before applying the hybridoma supernatants. In this protocol, cells are attached to a solid support (glass slides), which makes them easy to manipulate and transfer between different reagent solutions.

Antibodies: A Laboratory Manual, Second edition
Antibodies: A Laboratory Manual, Second edition
Antibodies: A Laboratory Manual, Second edition

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