Antibodies, A Laboratory Manual, Second Edition, Edited by Edward A. Greenfield



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Chromatin Immunoprecipitation

(Protocol summary only for purposes of this preview site)

This protocol details individual steps sequentially performed during the chromatin immunoprecipitation procedure and is ideally suited for the formaldehyde fixation of chromatin to highly abundant proteins. The initial steps of chromatin preparation and evaluation before the performance of the immunoprecipitation of the target antigen(s) are described in this protocol, followed by step-by-step guidance in the washing and elution of target antigen(s) and the reverse cross-linking of the protein-associated DNA fragments for purification, amplification, and sequencing purposes.

Antibodies: A Laboratory Manual, Second edition
Antibodies: A Laboratory Manual, Second edition
Antibodies: A Laboratory Manual, Second edition

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