Antibodies, A Laboratory Manual, Second Edition, Edited by Edward A. Greenfield



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Single-Cell Cloning by Limiting Dilution

(Protocol summary only for purposes of this preview site)

Cloning hybridoma cells by limiting dilution is the easiest of the single-cell-cloning techniques. Two approaches are given below, one rapid technique for generating cultures that are close to being single-cell-cloned and one for single-cell cloning directly. Even though every attempt is made to ensure that the cells are in a single-cell suspension before plating, there is no way to guarantee that the colonies do not arise from two cells that were stuck together. Therefore, limiting dilution cloning should be performed at least twice to generate a clonal population.

Antibodies: A Laboratory Manual, Second edition
Antibodies: A Laboratory Manual, Second edition
Antibodies: A Laboratory Manual, Second edition

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