Antibodies, A Laboratory Manual, Second Edition, Edited by Edward A. Greenfield



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Testing for Mycoplasma Contamination by Growth on Microbial Medium

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The first case of Mycoplasma infection was isolated from a cow with pleuropneumonia; the organism was designated pleuropneumonia-like organism (PPLO). Mycoplasma screening can be performed on agar plates or in broth culture. Cultures are grown under both aerobic and anaerobic conditions because some common strains of Mycoplasma prefer the lack of oxygen (Mycoplasma buccale, Mycoplasma faucium, M. orale, and M. salivarium). Mycoplasma colonies form a characteristic fried egg appearance on agar plates (see Fig. 1), and this is the diagnostic feature used to confirm mycoplasma contamination. The colonies are small and are most easily seen with an inverted microscope.

Antibodies: A Laboratory Manual, Second edition
Antibodies: A Laboratory Manual, Second edition
Antibodies: A Laboratory Manual, Second edition

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