Antibodies, A Laboratory Manual, Second Edition, Edited by Edward A. Greenfield



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Determining the Class and Subclass of Monoclonal Antibodies Using Antibody Capture on Anti-Immunoglobulin Antibody-Coated Plates

(Protocol summary only for purposes of this preview site)

As described in Protocol 25, it can be useful to know the class/subclass of the monoclonal antibody produced by a specific hybridoma. In this protocol, class/subclass-specific antibodies are used to capture the monoclonal antibody from hybridoma supernatant. If the antibody is clonal, it will only be bound by one anti-heavy-chain capture antibody and one anti-light-chain antibody. No antigen is required.

Antibodies: A Laboratory Manual, Second edition
Antibodies: A Laboratory Manual, Second edition
Antibodies: A Laboratory Manual, Second edition

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