Antibodies, A Laboratory Manual, Second Edition, Edited by Edward A. Greenfield



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Antibody Capture on Permeabilized Whole Cells Binding (Intracellular Staining by Flow Cytometry/FACS)

(Protocol summary only for purposes of this preview site)

The procedure described in Protocol 5 can also be adapted to screen for antibodies to internal proteins by permeabilizing the cells before applying the hybridoma supernatants. In using this technique, useful controls are positive and negative cell lines with primary and secondary antibodies as well as positive and negative cell lines with secondary antibody alone.

Antibodies: A Laboratory Manual, Second edition
Antibodies: A Laboratory Manual, Second edition
Antibodies: A Laboratory Manual, Second edition

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