Antibodies, A Laboratory Manual, Second Edition, Edited by Edward A. Greenfield



Table of Contents

Expand All | Contract All

Conjugation of Antibodies to Horseradish Peroxidase

(Protocol summary only for purposes of this preview site)

Antibodies conjugated with horseradish peroxidase are one of the most widely used bioreagents in the biological sciences. The following is a basic protocol for adding HRP to a thiolated antibody (see Protocol 5). Ideally, one can use the same antibody with protected SATA and begin at Step 4. This protocol can be adapted for use with different cross-linkers. Conjugation methods usually focus on linking through the lysines on HRP because there are only six of them and their modification does not adversely affect enzyme activity.

Antibodies: A Laboratory Manual, Second edition
Antibodies: A Laboratory Manual, Second edition
Antibodies: A Laboratory Manual, Second edition

Search for information about other protocols included in the book: