Antibodies, A Laboratory Manual, Second Edition, Edited by Edward A. Greenfield



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Antibody Capture on Nitrocellulose Membrane: High-Throughput Western Blot Assay for Hybridoma Screening

(Protocol summary only for purposes of this preview site)

In this procedure, proteins in solution or in a cell lysate are separated according to size by gel electrophoresis and then transferred by blotting to a nitrocellulose sheet. Antigen bound to the solid substrate is incubated with the primary antibody, and the resultant antibodyantigen complexes are detected by a horseradish peroxidase (HRP)conjugated secondary antibody and a chemiluminescent substrate for HRP.

Antibodies: A Laboratory Manual, Second edition
Antibodies: A Laboratory Manual, Second edition
Antibodies: A Laboratory Manual, Second edition

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