Antibodies, A Laboratory Manual, Second Edition, Edited by Edward A. Greenfield



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Intraperitoneal Injections without Adjuvant into Mice and Rats

(Protocol summary only for purposes of this preview site)

Intraperitoneal (i.p.) injections are the most commonly used method for introducing antigens into mice and rats. This can include using live cells as the antigen. The intended immunogen is typically cell-surface proteins. If it can be avoided, do not inject live viruses, bacteria, or fungi because the entire mouse colony might become infected. These organisms are commonly killed or inactivated before injection. Typically, it is not necessary to use adjuvant with whole-cell injections.

Antibodies: A Laboratory Manual, Second edition
Antibodies: A Laboratory Manual, Second edition
Antibodies: A Laboratory Manual, Second edition

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