Antibodies, A Laboratory Manual, Second Edition, Edited by Edward A. Greenfield



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Simple Multiplexed Antibody-Binding Assay

(Protocol summary only for purposes of this preview site)

This simple protocol tests antibody binding to target antigens on the surface of cells. This assay is powerful because negative controls are built into each well of the screening plates. It can be used for screening crude supernatants from hybridomas and bacterial periplasmic extracts when screening phage libraries. Using cell-permeant dyes allows the negative and positive cell lines to be color-coded and screened in the same well. A variant of the assay can be performed by bead-based ELISA when the target antigen is presented on beads.

Antibodies: A Laboratory Manual, Second edition
Antibodies: A Laboratory Manual, Second edition
Antibodies: A Laboratory Manual, Second edition

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