Antibodies, A Laboratory Manual, Second Edition, Edited by Edward A. Greenfield



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Counterstaining Cells

(Protocol summary only for purposes of this preview site)

Counterstains are used to help differentiate the various cell types or subcellular structures seen in cell staining. It is essential for tissue sections, allowing the identification of the cell types, but also may be helpful in other staining reactions. Counterstains should not be used with fluorochromes because the commonly used counterstains autofluoresce. To choose an appropriate counterstain, first determine whether the cell-staining detection reagent (often a chromogenic substrate) is soluble in alcohol.

Antibodies: A Laboratory Manual, Second edition
Antibodies: A Laboratory Manual, Second edition
Antibodies: A Laboratory Manual, Second edition

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