Antibodies, A Laboratory Manual, Second Edition, Edited by Edward A. Greenfield



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Standard Immunization of Rabbits

(Protocol summary only for purposes of this preview site)

Rabbits are given biweekly injections of a purified antigen, cultured cells, or cDNA. The injection sites on the rabbit are shaved and disinfected before immunization. Adjuvants are mixed with the immunizing antigen for the first two immunizations only. Complete Freund's adjuvant is only used with the first immunization. Subsequent immunizations are performed in phosphate-buffered saline (PBS) or normal saline, with or without Incomplete Freund's adjuvant.

Antibodies: A Laboratory Manual, Second edition
Antibodies: A Laboratory Manual, Second edition
Antibodies: A Laboratory Manual, Second edition

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