Antibodies, A Laboratory Manual, Second Edition, Edited by Edward A. Greenfield



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Counting Myeloma or Hybridoma Cells

(Protocol summary only for purposes of this preview site)

For most purposes, the number of hybridoma or myeloma cells can be estimated simply by observing the cells under the microscope. When an exact cell count is needed, the number can be determined by using a hemocytometer (improved Neubauer counting chambers are the most commonly used). This is a simple device in which a special coverslip rests on supports that hold it 0.1 mm above the base of the slide. The slide is engraved with a series of lines that form 1 1mm squares. By counting the number of cells within the 0.1-mm3 chamber formed by the 1 1mm square and the height of the coverslip, an accurate quantitation of cells per milliliter can be calculated.

Antibodies: A Laboratory Manual, Second edition
Antibodies: A Laboratory Manual, Second edition
Antibodies: A Laboratory Manual, Second edition

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