Antibodies, A Laboratory Manual, Second Edition, Edited by Edward A. Greenfield



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Antigen Capture in Solution: Immunoprecipitation

(Protocol summary only for purposes of this preview site)

Immunoprecipitation is rarely used for screening hybridoma fusions because the assays are tedious and time-consuming. However, it can be useful when working with complex antigens because the precipitated antigen is normally detected after SDSpolyacrylamide electrophoresis and thus it is simple to discriminate between true and false positives. Furthermore, the assay provides information regarding the molecular weight of the antigen.

Antibodies: A Laboratory Manual, Second edition
Antibodies: A Laboratory Manual, Second edition
Antibodies: A Laboratory Manual, Second edition

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