Antibodies, A Laboratory Manual, Second Edition, Edited by Edward A. Greenfield



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Detecting Alkaline PhosphataseLabeled Cells Using BCIP-NBT

(Protocol summary only for purposes of this preview site)

Bromochloroindoyl phosphate/nitro blue tetrazolium (BCIP/NBT) is the most commonly used of the chromogenic substrates for alkaline phosphatase. It is somewhat more sensitive than NABP/New Fuchsin but gives less contrast during microscopic observation.

Antibodies: A Laboratory Manual, Second edition
Antibodies: A Laboratory Manual, Second edition
Antibodies: A Laboratory Manual, Second edition

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