Antibodies, A Laboratory Manual, Second Edition, Edited by Edward A. Greenfield



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Preparing mFc- and hFc-Fusion Proteins from Mammalian Cells

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Fc-fusion proteins are composed of an immunoglobulin Fc domain that is directly linked to the antigen of interest. Fc-fusion proteins have several applications in protein microarrays, oncological therapies, and vaccine and antibody development. Presence of the Fc domain significantly increases the plasma life of the fusion partner, which prolongs therapeutic activity. Furthermore, the Fc domain enhances the solubility and stability of the partner molecule. Because Fc-fusion proteins are secreted into the culture medium, purification by affinity chromatography is relatively easy and cost effective. Immunizing a murine host with mFc-fusion protein generates an antigen-specific immune response because the Fc domain is recognized as self by the host.

Antibodies: A Laboratory Manual, Second edition
Antibodies: A Laboratory Manual, Second edition
Antibodies: A Laboratory Manual, Second edition

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