Antibodies, A Laboratory Manual, Second Edition, Edited by Edward A. Greenfield



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Testing for Mycoplasma Contamination Using Reporter Cells

(Protocol summary only for purposes of this preview site)

InvivoGen distributes a commercial kit, PlasmoTest, that uses mycoplasma sensor cells to detect the presence of mycoplasmas that lead to a color change of the HEK-Blue Detection medium. The mycoplasma sensor cells recognize mycoplasmas through Toll-like Receptor 2 (TLR2), a pathogen-recognition receptor that is part of the innate immune system. If there are mycoplasmas present, it will bind TLR2, initiating a signaling cascade that activates NF-B and other transcription factors. These transcription factors induce the secretion of SEAP (secreted embryonic alkaline phosphatase) in the supernatant, which results in a purple/blue coloration of the HEK-Blue Detection medium (Fig. 1).

Antibodies: A Laboratory Manual, Second edition
Antibodies: A Laboratory Manual, Second edition
Antibodies: A Laboratory Manual, Second edition

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