Antibodies, A Laboratory Manual, Second Edition, Edited by Edward A. Greenfield



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Iodination of Antibodies with Immobilized Iodogen

(Protocol summary only for purposes of this preview site)

Commercially available products make iodination of antibodies a simple and quick process. One example, available at Pierce, is the Iodination bead, or N-chloro-benzenesulfonamide immobilized on non-porous, polystyrene beads. This oxidizing agent was first reported by Markwell (1982) as a means for iodinating proteins.

  • WARNING: These methods use radioactive isotopes. Care must be used during experiments to limit exposure. Please consult your institution or the appropriate regulatory agency for proper disposal protocols.

Antibodies: A Laboratory Manual, Second edition
Antibodies: A Laboratory Manual, Second edition
Antibodies: A Laboratory Manual, Second edition

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