Antibodies, A Laboratory Manual, Second Edition, Edited by Edward A. Greenfield



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Preparing Frozen Tissue Sections

(Protocol summary only for purposes of this preview site)

Cell-staining studies on mammalian tissue are often performed on frozen sections. This is the gentlest method for the preparation of samples and gives good preservation of cell structure and antigens. Its principal disadvantages are that the specimens must be stored frozen and a special microtome, known as a cryostat, is required. In addition, many clinical specimens are not available in this form, and most classical histological descriptions of tissue structure and pathology are based on the use of formalin-fixed, paraffin-embedded (FFPE) material.

Antibodies: A Laboratory Manual, Second edition
Antibodies: A Laboratory Manual, Second edition
Antibodies: A Laboratory Manual, Second edition

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