Antibodies, A Laboratory Manual, Second Edition, Edited by Edward A. Greenfield



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Growing Adherent Cells on Tissue Culture Dishes

(Protocol summary only for purposes of this preview site)

For low-resolution work, such as crude antigen detection, hybridoma screening, or antibody titration, cells for staining can be grown on regular tissue culture dishes. In this case, the fixative used must not contain more than 50 acetone; otherwise, the dish will become crazed and optically opaque.

Antibodies: A Laboratory Manual, Second edition
Antibodies: A Laboratory Manual, Second edition
Antibodies: A Laboratory Manual, Second edition

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