Antibodies, A Laboratory Manual, Second Edition, Edited by Edward A. Greenfield



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Antibody Capture in Polyvinyl Chloride Wells: Enzyme-Linked Detection (Indirect ELISA)

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In this antibody capture assay, the antigen is immobilized on a solid substrate (the surface of the wells in a polyvinyl chloride [PVC] microtiter plate), and antibodies in the hybridoma tissue culture supernatant are incubated with the antigen. Unbound antibodies are removed by washing, and antibodyantigen complexes are detected by secondary antibody conjugated to alkaline phosphatase (AP), which catalyzes the conversion of a chromogenic substrate to a blue/green product.

Antibodies: A Laboratory Manual, Second edition
Antibodies: A Laboratory Manual, Second edition
Antibodies: A Laboratory Manual, Second edition

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