Antibodies, A Laboratory Manual, Second Edition, Edited by Edward A. Greenfield



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Preparing Splenocyte Feeder Cell Cultures

(Protocol summary only for purposes of this preview site)

Although splenocyte feeder cell cultures can be used immediately, they are most effective when they are prepared 1 d before the single-cell cloning. Because spleen cells do not grow in normal tissue culture conditions, they are lost during the subsequent expansion of the hybridoma cells. Use a female mouse of the same genetic background as your hybridoma.

Antibodies: A Laboratory Manual, Second edition
Antibodies: A Laboratory Manual, Second edition
Antibodies: A Laboratory Manual, Second edition

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