Antibodies, A Laboratory Manual, Second Edition, Edited by Edward A. Greenfield



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Harvesting Spleens from Mice, Rats, and Hamsters

(Protocol summary only for purposes of this preview site)

Once the immunized animal has developed a serum antibody titer sufficient for hybridoma fusion, a splenectomy is performed. This easy-to-perform procedure allows for the harvest of a large number of potential antibody-producing B lymphocytes.

Antibodies: A Laboratory Manual, Second edition
Antibodies: A Laboratory Manual, Second edition
Antibodies: A Laboratory Manual, Second edition

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