Antibodies, A Laboratory Manual, Second Edition, Edited by Edward A. Greenfield



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Sampling Rat Serum from the Tail Vein

(Protocol summary only for purposes of this preview site)

Obtaining blood by superficially nicking the vessel perpendicular to the tail is easily performed in rats but produces a sample of variable quality that may be contaminated with tissue products. Sample quality decreases and hemolysis may occur with prolonged bleeding times and milking of the tail. Blood collection should not exceed 79 of the animal's total blood volume or 1 of total body weight every 24 wk.

Antibodies: A Laboratory Manual, Second edition
Antibodies: A Laboratory Manual, Second edition
Antibodies: A Laboratory Manual, Second edition

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