Antibodies, A Laboratory Manual, Second Edition, Edited by Edward A. Greenfield



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Injecting Mice Intravenously

(Protocol summary only for purposes of this preview site)

The most direct route of injection for soluble antigens without adjuvant is intravenous (i.p.), allowing efficient delivery of the antigen to lymphoid organs like the spleen and lymph nodes. However, there is a risk of anaphylactic shock, which can be prevented by administering a prior injection of antihistamine. There is also no depot effect without the adjuvant present. The veins on the lateral sides of the mouse's tail are easily accessed for i.v. injection. These veins are for thermoregulation. Applying a mild form of heat (via a heat lamp) will cause the veins to dilate in order to disseminate heat, facilitating the injection. The mouse should be restrained so that its tail is accessible.

Antibodies: A Laboratory Manual, Second edition
Antibodies: A Laboratory Manual, Second edition
Antibodies: A Laboratory Manual, Second edition

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