Antibodies, A Laboratory Manual, Second Edition, Edited by Edward A. Greenfield



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Lysing Yeast Cells with Glass Beads

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Yeast cells display cell walls that must first be broken before the addition of detergents for lysis (Garrido et al. 1994; Horvath and Riezman 1994; Harlow and Lane 2006a,b; Wong et al. 2007; Lim et al. 2008; Simpson 2011). This method describes the use of glass beads in combination with a mechanical bead beater to disrupt cell walls of both Saccharomyces cerevisiae or Schizosaccharomyces pombe directly in a nonionic detergent Lysis buffer containing 0.1 Nonidet P-40. Alternatively, this protocol can be applied for the lysis of yeast cells in Lysis buffer without detergent and upon completion of the bead beating; Triton X-100 is added to complete lysis.

Antibodies: A Laboratory Manual, Second edition
Antibodies: A Laboratory Manual, Second edition
Antibodies: A Laboratory Manual, Second edition

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