Antibodies, A Laboratory Manual, Second Edition, Edited by Edward A. Greenfield



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Purification of Antibodies: Low-pH DEAE Chromatography

(Protocol summary only for purposes of this preview site)

When serum containing antibodies is applied to DEAE at a slightly acidic pH, the IgG flows through the column while most other serum proteins bind to the DEAE. This method is best performed using a batch method. The DEAE beads can be kept in a disposable syringe containing a polypropylene frit, a glass reactor containing a coarse-sintered glass frit, or other suitable vessel.

Antibodies: A Laboratory Manual, Second edition
Antibodies: A Laboratory Manual, Second edition
Antibodies: A Laboratory Manual, Second edition

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