Antibodies, A Laboratory Manual, Second Edition, Edited by Edward A. Greenfield



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Ammonium Sulfate Fractionation of Antibodies

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The addition of ammonium sulfate reduces the effective solubility of proteins through direct competition for binding sites on the surface of the protein. The resulting precipitated proteins can be isolated by centrifugation. An ammonium sulfate concentration between 40 and 50 results in the precipitation of IgG from most species, and thus 50 is usually used. Because other proteins can be trapped within the aggregated protein, the use of ammonium sulfate does not result in a purified antibody fraction and as such should be considered a first step in a multistep antibody purification protocol.

Antibodies: A Laboratory Manual, Second edition
Antibodies: A Laboratory Manual, Second edition
Antibodies: A Laboratory Manual, Second edition

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