Antibodies, A Laboratory Manual, Second Edition, Edited by Edward A. Greenfield



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Lysing Yeast

(Protocol summary only for purposes of this preview site)

After yeast cells have been treated with paraformaldehyde, the cell wall removed by enzymatic digestion, and the spheroplasts attached to poly-L-lysine coated slides (all performed in Protocol 6), they are ready for methanol fixation.

Antibodies: A Laboratory Manual, Second edition
Antibodies: A Laboratory Manual, Second edition
Antibodies: A Laboratory Manual, Second edition

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