Antibodies, A Laboratory Manual, Second Edition, Edited by Edward A. Greenfield



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Staining the Blot for Total Protein with Ponceau S

(Protocol summary only for purposes of this preview site)

Ponceau S is the more common staining method in immunoblotting protocols because it is compatible with antibodyantigen binding, is cost efficient, and provides a good contrast between the stained bands and background. In this protocol, nitrocellulose or PVDF membrane is rinsed with ultrapure H2O after the transfer of proteins. Ponceau S dye is applied as an acidic aqueous solution, and the proteins on the membrane are stained with red color. The membrane is briefly destained with water and can be photographed or scanned to obtain the image of the total protein staining. Individual lane positions or the molecular weight standards can be marked with a pencil, if required.

Antibodies: A Laboratory Manual, Second edition
Antibodies: A Laboratory Manual, Second edition
Antibodies: A Laboratory Manual, Second edition

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