Antibodies, A Laboratory Manual, Second Edition, Edited by Edward A. Greenfield



Table of Contents

Expand All | Contract All

Antigen Capture in 96-Well Plates (Capture or Sandwich ELISA)

(Protocol summary only for purposes of this preview site)

In this assay, the polyvinyl chloride (PVC) wells of a high-binding-capacity ELISA plate are first coated with an affinity-purified rabbit anti-mouse immunoglobulin and then incubated with hybridoma tissue culture supernatant. Monoclonal antibodies in the supernatant are captured on the coated PVC surface and detected by screening with biotin- or histidine (His)tagged antigen. The procedure was adapted from Wide and Porath (1966), Catt and Tregear (1967), Engvall and Perlmann (1971a,b), and Van Weemen and Schuurs (1971).

Antibodies: A Laboratory Manual, Second edition
Antibodies: A Laboratory Manual, Second edition
Antibodies: A Laboratory Manual, Second edition

Search for information about other protocols included in the book: