Antibodies, A Laboratory Manual, Second Edition, Edited by Edward A. Greenfield



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cDNA Immunization of Mice, Rats, and Hamsters

(Protocol summary only for purposes of this preview site)

DNA or genetic immunization has been useful in vaccine technology and can also be used to generate immune responses to novel proteins. It is particularly useful when a properly folded protein is difficult to isolate or make in recombinant form and the required antibody must recognize a conformational epitope. Injecting cDNA allows the protein to be expressed in native form by the animal's own cells in vivo and then presented to the immune system.

Antibodies: A Laboratory Manual, Second edition
Antibodies: A Laboratory Manual, Second edition
Antibodies: A Laboratory Manual, Second edition

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