Antibodies, A Laboratory Manual, Second Edition, Edited by Edward A. Greenfield



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Direct Competitive ELISA

(Protocol summary only for purposes of this preview site)

The competitive ELISA (cELISA; also called an inhibition ELISA) is designed so that purified antigen competes with antigen in the test sample for binding an antibody that has been immobilized in microtiter plate wells (Jones 1992; Kittelberger et al. 2011). The same concept works if the immobilized molecule is antigen and the competing molecules are purified labeled antibody versus antibody in a test sample. Direct cELISAs incorporate labeled antigen or antibody, whereas indirect assay configurations (Protocol 6) use reporter-labeled secondary antibodies. The cELISA is very useful for determining the concentration of small-molecule antigens in complex sample mixtures (Darwish et al. 2011).

Antibodies: A Laboratory Manual, Second edition
Antibodies: A Laboratory Manual, Second edition
Antibodies: A Laboratory Manual, Second edition

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